Composite

Part:BBa_K3453114:Design

Designed by: Maeva Cherriere   Group: iGEM20_Evry_Paris-Saclay   (2020-10-04)


DmMatK Toehold Switch 2.1 with sfGFP-LVAtag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 38
    Illegal BamHI site found at 79
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 141
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

T7 promoter (BBa_K2150031) + DmMatK Toehold Switch 2.1 (BBa_K3453014) + sfGFP-LVAtag coding sequence (BBa_K2675006) + the strong SBa_000587 Terminator (BBa_K3453000)


Source

DNA synthesis & Assembly

References